W1018-53
West Nile Virus. NS3 Protease (WNV)
Size
£847.00
- SKU:
- W1018-53
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ser1423-Lys1470, Gly1506-Leu1689 from wnvNS3 Protease, expressed in E coli.
- Clone:
- 8H153
- Format:
- Supplied as a lyophilized powder in TBS, 5% trehalose. Reconstitute with 200ul sterile TBS.
- Extra Details:
- NS3 and NS2b are non-structural viral proteins that play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 is a trypsin-like serine protease that requires NS2b as a cofactor. Applications: Suitable for use in Western Blot, Neutralization, Immunoprecipitation and Direct ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml using recombinant viral wnv NS3 Protease Neutralization: Measured by its ability to neutralize Recombinant Viral wnvNS3 Protease (0.5ug/ml) cleavage of the fluorogenic peptide substrate pERTKR-Amc (20uM). The Neutralization Dose (ND50) is typically 15ug/ml. Immunoprecipitation: 25ug/mL using conditioned cell culture medium spiked with recombinant viral wnvNS3 Protease. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder and reconstituted products must be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Reconstitute with sterile PBS. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice