V4990

VspI

Size

£301.00

SKU:: V4990
Storage Conditions:: -20°C
Supplier:: United States Biological
Extra Details:: 5'-A T^T A A T-3' 3'-T A A T^T A-5' Enzyme Properties: Stability during Prolonged Incubation: A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of DNA in 16 hours at 37°C. Thermal Inactivation: Enzyme is inactivated by incubation at 65°C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Compatible Ends: Csp6I, MaeI, NdeI, Tru1I Number of Recognition Sites in DNA: Lambda: 17 PhiX174: 2 M13mp18/19: 7 pBR322: 1 pUC18/19: 3 pUC57: 3 pTZ19R/U: 3 pBluescriptIIKS(-/+): 3 pBluescriptIISK(-/+): 3 pACYC177: 2 pACYC184: 1 Supplied With: Provided to simply buffer selection. R1625: Restriction Enzyme Buffer A, 10X. R1625-03: Restriction Enzyme Buffer D, 10X. Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.