V4880
VSIG4 (V-set and Ig Domain-containing Protein 4, Z39Ig)
Size
£895.00
- SKU:
- V4880
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Arg20-Pro283 from human VSIG4, expressed in NS0 cells.
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- VSIG4 (V-set and Ig domain-containing protein 4; also Z39Ig) is a 45-50kD macrophage-specific protein that belongs to the B7 family of molecules. Its constitutive expression depresses unwarranted T cell activation. Human VSIG4 is 399 amino acids (aa) in length. It is a type I transmembrane (TM) glycoprotein that contains a 264 aa extracellular domain (ECD) (aa 20-283) and a 95 aa cytoplasmic region. The ECD contains one V-type (aa 21-131) and one C2-type Ig-like domain (aa 143-226). Mouse VSIG4 has no C2-type domain. Over aa 20-139, human VSIG4 is 82% aa identical to mouse VSIG4. Multiple splice forms exist in human that impact the ECD and TM segments. One isoform deletes aa 67-143, while two additional isoforms substitute two aa’s for aa 138-233. Two new isoforms remove all or part of the TM region. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1ug/ml with recombinant human VSIG4 Fc Chimera Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice