V2700-15
VWA1 (von Willebrand Factor A Domain-containing Protein 1, WARP)
Size
£787.00
- SKU:
- V2700-15
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Arg19-Pro445 from human WARP, expressed in NS0 cells.
- Clone:
- 10K63
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Von Willebrand factor A (vWFA) domain-related protein (WARP) is a 50kD glycoprotein member of the vWFA domain superfamily of extracellular matrix proteins. It is expressed in embryonic articular cartilage, skeletal muscle and basement membranes in the PNS. WARP forms disulfide-linked homodimers and multimers, and complexes with perlecan. Secreted human WARP contains a vWFA domain (aa34-213), two fibronectin type III domains (aa211-301 and 331-421) that likely bind to the GAG modification of perlecan, and one potential site for N-linked glycosylation. There is one alternate start site at Met213. Mature human WARP is 72% aa identical to mature mouse and rat WARP. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml, under reducing conditions Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice