Vitamin D Binding Protein (VDBP, VDB, DBP, DBP/GC, Gc Globulin, Group Specific Component, Group-specific Component, GC, hDBP, Vitamin D Binding alpha Globulin)

Size

£825.00

SKU:: V2130-12
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Leu17-Ser476 from mouse Vitamin D Binding Protein, expressed in NS0 cells.
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: VDBP (Vitamin D binding protein; also group-specific component and GC-globulin) is a 52-58kD, monomeric glycoprotein member of the ALB/AFP/VDB family of molecules. It is found in blood, urine and CSF, carries Vitamin D and its metabolites, and serves as an actin-scavenging protein. Mature mouse VDBP is 460 amino acids (aa) in length. It contains three albumin-type domains (aa 26-476) that are accompanied by 14 intrachain disulfide bonds. There are three potential alternative splice forms. One shows a deletion of aa 346-421, a second shows a 67 aa substitution for aa 345-421, and a third shows a 34 aa substitution for aa 346-423. All these variants involve the second and third albumin-like domains. Mature mouse VDBP (aa 17-476) is 77% and 90% aa identical to human and rat VDBP, respectively. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml in lysates of mouse liver Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice