Vimentin (FLJ36605, VIM) (Not for Export EU)

Size

£871.00

SKU:: V2122-12
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Guinea Pig
Immunogen:: Vimentin purified from calf lens
Format:: Supplied as a liquid, 0.5% BSA, 0.09% sodium azide
Extra Details:: Vimentin is an intermediate filament with a molecular weight of ~58kD, and an average diameter of 10 nm. Within the same species, the central domain of the filament is 30% homologous to cytokeratin, desmin, neurofilaments, and glial fibrillary acidic protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. It is usually co-expressed with cytokeratin in some epithelial cells such as Sertoli cells. Vimentin expression may change as a tumor becomes more aggressive, as in the case of hyperplastic prostate where prostatic intraepithelial neoplasia strongly expresses vimentin (91%), but invasive carcinoma is minimally reactive with Mouse anti-Vimentin. Vimentin is useful as part of an antibody panel for differential diagnostic purposes. It may be included in an antibody panel for differentiating soft tissue tumors. For example, co-expression of vimentin and cytokeratin is indicative of epitheloid sarcoma. In one case, a differential antibody panel using vimentin, cytokeratin, CEA, and EMA distinguished reactive mesothelial cells from malignant epithelial cells in the cytodiagnosis of serous effusions in 39/46 cases (85%). Applications: Suitable for Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen): 1:100 Immunohistochemistry (Paraffin): 1:50. Microwave treatment is recommended. Western Blot: 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice