V2121-87J

VGAT (Vesicular GABA Amino Acid Transporter, Vesicular GABA Transporter, GABA and Glycine Transporter, hVIAAT, Solute Carrier Family 32 (GABA Vesicular Transporter) Member 1, Solute Carrier Family 32 Member 1, SLC32A1, Vesicular Inhibitory Amino Acid Transporter, VIAAT)

Size

£696.00

SKU:: V2121-87J
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat VGAT, conjugated to keyhole limpet hemocyanin, conjugated to KLH. Species sequence homology: bovine, canine and non-human primate
Format:: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Extra Details:: The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005). Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry: 1:100-1:1000 Immunocytochemistry: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice