Vesicular Acetylcholine Transporter (VAChT) (Not for Export EU)

Size

£1,161.00

SKU:: V2121-85B
Application:: IHC-Fr
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide from the C-terminus of human VAChT conjugated to BSA.
Format:: Supplied as a lyophilized powder.
Extra Details:: The antiserum against the vesicular acetylcholine transporter is a unique immunohistochemical marker for cholinergic nerves, more specific than the commonly used acetylcholinesterase (AchE), since it does not react with postsynaptic neurons, and is more sensitive than choline acetyltransferase (ChAT). Applications: Suitable for use in Immunohistochemistry (frozen sections). Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:800-1:1200 using FITC conjugates as secondary antibodies; Incubation Time Overnight at 2-8°C. Optimal dilution determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.