V2121-60B
Verotoxin I-B subunit (Shiga-like, SLT-1b)
Size
£1,068.00
- SKU:
- V2121-60B
- Purity:
- Purified by Protein G affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified, biologically active SLT from E. coli H30
- Clone:
- 9G300 (13C4)
- Format:
- Supplied as a liquid in PBS, 0.1% BSA.
- Extra Details:
- Verotoxins are also called Shiga-like toxins (SLTs). Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11 and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia and micro- angiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC. SLT-I and Shiga toxin share > 99% deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60% deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct. The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis. Applications: Suitable for use in Western Blot, ELISA and Inhibition. Other applications not tested. Recommended Dilution: Western Blot: 1:10 Inhibition: in vitro dilutions should be made according to the amount of toxin to be inactiviated. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice