V2120-80
Vasohibin
Size
£748.00
- SKU:
- V2120-80
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Pro2-Val365 from human Vasohibin isoform 1, expressed in NS0 cells.
- Clone:
- 8H175
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Vasohibin is an endothelium-derived negative feedback regulator of angiogenesis. The 365 aa, 44kD human Vasohibin shows 93% aa identity with mouse Vasohibin. Vasohibin has no canonical signal sequence, but the full-length form is only associated with cells, while N-terminally cleaved, active 42kD and 36kD forms are both cell-associated and secreted. Minor amounts of C-terminally cleaved 27kD form are inactive and lack the C-terminal heparin-binding basic domain. Vasohibin expression is upregulated by VEGF and FGF2, but this can be modulated by IL-1, TNFalpha or IFNy. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml with recombinant human Vasohibin. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice