V2110-18C
VEGF Receptor 2 (CD309 antigen, Fetal liver kinase 1, FLK1, KDR, Kinase insert domain receptor, KRD1, Ly73, Protein tyrosine kinase receptor FLK1, Vascular endothelial growth factor receptor 2, VEGFR, VEGFR2)
Size
£581.00
- SKU:
- V2110-18C
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to residues near the carboxy- terminal sequence of human VEGF receptor 2
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol
- Extra Details:
- Vascular endothelial growth factor receptor 2 (VEGFR-2, KDR, Flk-1) is a major receptor transducing the effects of VEGF into endothelial cells. Upon ligand binding, VEGFR-2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of the adaptor proteins, including Shc, GRB2, PI3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The signaling of VEGFR-2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and prouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (4). Applications: Suitable for use in Western Blotting. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice