VPS35 (Vacuolar Protein Sorting-associated Protein 35, hVPS35, Maternal-embryonic 3, Vesicle Protein Sorting 35, MEM3, TCCCTA00141)

Size

£642.00

SKU:: V0650-24C
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-SPESEGPIYEGLIL, from human VPS35, at C-terminal (NP_060676.2).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: VPS35, otherwise known as MEM3, is a ubiquitously expressed member of the vacuolar protein sorting (VPS) family, and vital component of the multimeric retromer complex, involved in the retrieval of lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans Golgi network. Applications: Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.1-1ug/ml, observed in human brain cerebellum lysates on ~85kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.