VPS25 (Vacuolar Protein-sorting-associated Protein 25, hVps25, Dermal Papilla-derived Protein 9, ELL-associated Protein of 20kD, ESCRT-II Complex Subunit VPS25, DERP9, EAP20)

Size

£642.00

SKU:: V0649-88B
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-QPNVDTRQKQ, from human VPS25, at the internal region of the protein (NP_115729.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: VPS25 is a component of the ESCRT-II complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:1000 Western Blot: 0.5-1.5ug/ml, observed in human duodenum, kidney and liver lysates on ~20-22kD bands Immunohistochemistry (Paraffin): 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.