V0500-00V-ML650

V5 Epitope Tag GKPIPNPLLGLDST (Pk-tag, Paramyxovirus, SV5) (MaxLight 650)

Size

£741.00

SKU:: V0500-00V-ML650
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Paramyxovirus SV5, Simian-Virus 5.
Clone:: 3H3115 (SV5-Pk1)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Extra Details:: MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence. The V5 epitope tag is a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Applications: Suitable for use in RIA, Immunofluorescence, Flow Cytometry, FLISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Hybridoma: SP2/0 Ag14 myeloma cells with spleen cells from Balb/c mice. Recommended Secondary Antibodies: I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb xMo I1904-66C: IgG, F(ab’)2X-adsorbed (HRP) Pab Rb xMo Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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