V0500-00T7

V5 Epitope Tag GKPIPNPLLGLDST (Pk-tag, Paramyxovirus, SV5)

Size

£524.00

SKU:: V0500-00T7
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: A recombinant peptide corresponding to the PK tag fused to a class II-restricted T helper cell epitope.
Clone:: 9i125 (SV5-Pk4)
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: V5 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.