U1100-08

UFC1, Recombinant, Human, aa1-167, His-Tag (HSPC155)

Size

£607.00

SKU:: U1100-08
Molecular Weight:: 21.6
Purity:: ~90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl buffer, pH8.0, 1mM DTT, 0.1M sodium chloride, 20% glycerol.
Extra Details:: UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1. UFM1 is activated by a novel E1-like enzyme, UBA5, by forming a high-energy thioester bond. Activated UFM1 is then transferred to its cognate E2-like enzyme, UFC1, in a similar thioester linkage. Source: Recombinant corresponding to aa1-467 from human UFC1, fused to His-tag at N-terminus expressed in E.coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MADEATRRVV SEIPVLKTNA GPRDRELWVQ RLKEEYQSLI RYVENNKNAD NDWFRLESNK EGTRWFGKCW YIHDLLKYEF DIEFDIPITY PTTAPEIAVP ELDGKTAKMY RGGKICLTDH FKPLWARNVP KFGLAHLMAL GLGPWLAVEI PDLIQKGVIQ HKEKCNQ Molecular Weight: ~21.6kD (187aa), confirmed by MALDI-TOF Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.