U1000-95

Ubiquitin Conjugating Enzyme E2S, Recombinant Human (UBE2S)

Size

£422.00

SKU:: U1000-95
Molecular Weight:: 27.9
Purity:: ~90% by SDS-PAGE Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as liquid in 20 mM Tris-HCl buffer, pH8.0, 1mM DTT and 20% glycerol
Extra Details:: UBE2S, also known as ubiquitin-conjugating enzyme E2S, is a member of the ubiquitin-conjugating enzyme family. This protein is able to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. It catalyzes the covalent attachment of ubiquitin to other proteins. Recombinant UBE2S protein, fused to His-tag at N-terminus, , expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant human UBE2S, aa1-222, His-tagged, expressed in E. coli AA Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNSN VENLPPHIIR LVYKEVTTLT ADPPDGIKVF PNEEDLTDLQ VTIEGPEGTP YAGGLFRMKL LLGKDFPASP PKGYFLTKIF HPNVGANGEI CVNVLKRDWT AELGIRHVLL TIKCLLIHPN PESALNEEAG RLLLENYEEY AARARLLTEI HGGAGGPSGR AEAGRALASG TEASSTDPGA PGGPGGAEGP MAKKHAGERD KKLAAKKKTD KKRALRRL Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice