U1000-90A
Ubiquitin Conjugating Enzyme E2L6 (UBE2L6, UbcH8)
Size
£484.00
- SKU:
- U1000-90A
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant human UBE2L6 (1-152aa) purified from E. coli
- Clone:
- 10C318
- Format:
- Supplied in liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- Ubiquitin-conjugating enzyme E2L6 (UBE2L6), also known as UbcH8, is a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination of a protein substrate requires the concerted action of 3 classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin protein ligases. The E2 ubiquitin-conjugating enzyme is highly similar in primary structure to the enzyme encoded by UBE2L3 gene. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:3000 immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice