U1000-86S

UBE2D1, Recombinant, Human, aa1-147, His-Tag (Ubiquitin-conjugating Enzyme E2-D1, Ubiquitin-protein Ligase D1, Ubiquitin Carrier Protein D1, UbcH5, Ubiquitin-conjugating Enzyme E2-17kD 1, Ubiquitin-conjugating Enzyme E2(17)KB 1, UBC4/5 Homolog, Stimulator of Fe Transport, SFT, SFT, UBC5A, UBCH5, UBCH5A)

Size

£512.00

SKU:: U1000-86S
Molecular Weight:: 19
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl buffer, pH8, 0.1M sodium chloride, 40% glycerol.
Extra Details:: UBE2D1 belongs to the ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. UBE2D1 is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Purified by using conventional chromatography. Source: Recombinant corresponding to aa1-147 of human UBE2D1, fused to His-tag at N-terminus, expressed in E.coli. AA Sequences: MGSSHHHHHH SSGLVPRGSH MGSMALKRIQ KELSDLQRDP PAHCSAGPVG DDLFHWQATI MGPPDSAYQG GVFFLTVHFP TDYPFKPPKI AFTTKIYHPN INSNGSICLD ILRSQWSPAL TVSKVLLSIC SLLCDPNPDD PLVPDIAQIY KSDKEKYNRH AREWTQKYAM Molecular Weight: ~19kD (170aa), confirmed by MALDI-TOF Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice