U1000-86P

UBE2D2, Recombinant, Human, aa1-147, His-Tag (Ubiquitin-conjugating enzyme E2 D2, E2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH5B)

Size

£512.00

SKU:: U1000-86P
Molecular Weight:: 18.9
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8, 1mM DTT, 0.1M sodium chloride, 10% glycerol.
Extra Details:: UBE2D2 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. UBE2D2 catalyzes ubiquitination of IkB-alpha in a phosphorylation and SCFB-TRCP dependent manner. Purified by using conventional chromatography techniques. Source: Recombinant corresponding to aa1-147 of human UBE2D2, fused to His-tag at N-terminal, expressed in E.coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM Molecular Weight: ~18.9kD (167aa), confirmed by MALDI-TOF Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.