U1000-86N

Ubiquitin Conjugating Enzyme E2 D1, N-terminal His-Tag, Recombinant, Human (E2(17)KB1, SFT, Stimulator of Fe transport, UBC4/5, UBC4/5 homolog, UbcH5, UBCH5, UbcH5A, UBCH5A, Ubiquitin carrier protein D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-conjugating enzyme E2-17kD 1, Ubiquitin-protein ligase D1)

Size

£779.00

SKU:: U1000-86N
Molecular Weight:: 17
Purity:: Purified ≥80%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% tween-20, 3mM DTT, 20% glycerol.
Extra Details:: Source: Recombinant corresponding to aa2-end of human UbcH5a with N-terminal HIS tag expressed in E. coli (NM_003338). Specific Activity: 50ul reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, UBE1 (0.5ug), UbcH5a or other E2, Biotin-Ubiquitin (0.5ug) and Smurf2 (2) in 50mM Tris pH 7.4 for 30 min at 37°C, then add to streptavidin coated plates for 30 min at 37°C. Incubate with anti-Smurf2, then Europium-labeled secondary antibody, and detect by time resolved fluorescence (lambda-ex=330nm, lambda-em=620nm). Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.