T9300-08B
TWIST2, Control Peptide (Twist-related Protein 2, Class A Basic Helix-loop-helix Protein 39, bHLHa39, Dermis-expressed Protein 1, Dermo-1, BHLHA39, DERMO1)
Size
£416.00
- SKU:
- T9300-08B
- Molecular Weight:
- 3
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
- Extra Details:
- Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by Twist gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Twist. It is thought that during osteoblast development this protein may inhibit osteoblast maturation and maintain cells in a preosteoblast phenotype. Twist and Dermo1, also called Twist1 and Twist2 respectively, were induced by a cytokine signaling pathway that required the dorsal-related protein Rela, a member of the nuclear factor kappa-B family of transcription factors, in mice. Twist1 and Twist2 repressed cytokine gene expression through interaction with Rela. TWIST proteins regulate cytokine signaling by establishing a negative feedback loop that represses the NFKB-dependent cytokine pathway. Source: Synthetic peptide corresponding to 20aa of mouse Twist2, N-terminal. Molecular Weight: ~3kD Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested. Recommended Dilution: ELISA: 5-10ug control peptide per 1ug of aff pure IgG or 1ul antiserum) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice