T9237-15A

Tyrosine Hydroxylase (Tyrosine 3-monooxygenase, TH, Tyrosine 3-hydroxylase)

Size

£512.00

SKU:: T9237-15A
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Rat tyrosine hydroxylase.
Clone:: 10K388
Format:: Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.01% sodium azide. Reconstitute with 1ml sterile PBS.
Extra Details:: Tyrosine hydroxylase is involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Human TH gene contains 13 primary exons and spans approximately 8 kb. TH is in the 11p15.5 region. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.25-0.5ug/ml Immunohistochemistry (FFPE): 0.5-1ug/ml Immunohistochemistry (Frozen): 0.5-1ug/ml using formalin or acetone fixed tissues Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.