T9237-10C
Tyrosine Hydroxylase, phosphorylated (Ser40) (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)
Size
£771.00
- SKU:
- T9237-10C
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser40 of rat tyrosine hydroxylase (KLH)
- Format:
- Supplied as a liquid in 10mM Hepes, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
- Extra Details:
- Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982; Haycock et al., 1992; Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs. Applications: Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:500 Immunocytochemistry: 1:50-1:1000 Immunohistochemistry: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice