T9237-10B
Tyrosine Hydroxylase, phosphorylated (Ser40) (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)
Size
£639.00
- SKU:
- T9237-10B
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phosphopeptide corresponding to the sequence surrounding Ser40 of human tyrosine hydroxylase. Species sequence homology (100%): human and mouse.
- Format:
- Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
- Extra Details:
- Tyrosine hydroxylase catalyzes the rate limiting step in the synthesis of dopamine and other catecholamines. The enzyme’s activity is regulated at the levels of transcription, translation and post-translational modification. Tyrosine hydroxylase exists in several different isoforms ranging from 50kD to about 62kD in human. The N-terminal regulatory domain contains three phospho-serine sites: Ser19, Ser31 and Ser40. Phosphorylation at Ser40 by PKA positively regulates the enzyme catalytic activity of the enzyme. Applications: Suitable for use in Immunofluorescence, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunofluorescence (IF-IC): 1:400 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice