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T9228-75C

Tyrosine (Tyr), phosphorylated

Size

£456.00

SKU:
T9228-75C
Purity:
Purified.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Synthetic phospho-Tyr-containing peptides
Clone:
3A42(P-Tyr-102)
Format:
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Extra Details:
Since the original observations that protein tyrosine kinases (PTKs) play critical roles in oncogenic transformation (1,2), it has become clear that both protein tyrosine kinases and phospho-tyrosine phosphatases control a wide range of critical biological processes (3,4). Antibodies specific for phospho-tyrosine (5,6) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies provide exceptionally sensitive new tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Other applications have not been tested. Recommended Dilution: Western Blot: 1:2000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 ELISA: 1:1000 Flow Cytometry: 1:400 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice