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T9228-75A

Tyrosine, phosphorylated (Tyr) (Agarose)

Size

£492.00

SKU:
T9228-75A
Application:
IP
Purity:
Purified.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Phospho-Tyr-containing peptides
Clone:
3A45(P-Tyr-100)
Format:
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Labeled with Agarose.
Extra Details:
Since the original observations that protein tyrosine kinases (PTKs) play critical roles in oncogenic transformation (1,2), it has become clear that both protein tyrosine kinases and phospho-tyrosine phosphatases control a wide range of critical biological processes (3,4). Antibodies specific for phospho-tyrosine (5,6) have been invaluable reagents in these studies. Exceptionally sensitive new tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. Applications: Suitable for use in Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunoprecipitation: 1:20; 20ul of 50% bead slurry to 200ul of cell lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice