T9162-76-ML490
Tumor Necrosis Factor Receptor 2, Soluble (sTNFR2) (MaxLight 490)
Size
£1,042.00
- SKU:
- T9162-76-ML490
- Application:
- FLISA
- Purity:
- Purified by Protein A affinity chromatography
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified recombinant human soluble TNF Receptor 2.
- Clone:
- 2B2.106
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. TNF-RII is expressed on a variety of human tissues including lymphocytes, monocytes, granulocytes, fibroblasts, and endothelial cells. In addition, TNF-RII is released from neutrophils and monocytes after activation. TNF-RII, a member of TNF receptor family, binds to TNF-a and TNF-b. The cytoplasmic domain of TNF-RII interacts with TRAF1 and TRAF2. Applications: Suitable for use in FLISA. Other applications not tested. Recommended Dilutions: FLISA (Capture): Dilute in an appropriate coating buffer to a concentration of 1-5ug/ml and use 100ul to coat each well. Further dilutions may be made in medium or buffered solution with carrier protein, such as PBS with 0.1-1.0% BSA. Optimal dilutions to be determined by the researcher. Hybridoma: BALB/c splenocytes were fused with NSO mouse myeloma cells. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice