T9161-56N
Tumor Necrosis Factor Receptor Associated Factor 3, NT (TRAF-3)
Size
£655.00
- SKU:
- T9161-56N
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide TRAF3 15aa near the amino terminus of human TRAF3 (Genbank accession No. NP_663777).
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
- Extra Details:
- Tumor necrosis factor (TNF) receptor associated factors (TRAFs) are the major signal transducers for the TNF receptor superfamily and the interleukin-1 receptor/Toll- like receptor (IL-1/TLR) superfamily (1). TRAF3 was first identified by its interaction with CD40 and the Epstein-Barr virus transforming protein LMP1 (2,3). Several TRAF3 mRNA splice variants exist and some of these can activate the transcription factor NF-κB (4). Besides CD40, TRAF3 also interacts with the TRFR superfamily member lymphotoxin-β receptor (LTβR) in association with TRAF2 and the apoptosis inhibitors cIAP1 and Smac. It has been suggested that TRAF3 induces mitochondria-mediated apoptosis upon binding of the TNF family cytokine LIGHT by LTβR (5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug’ml Optimal dilutions to be determined by the researcher. Positive Control: HeLa cell lysate Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice