T9160-36S

Tumor Necrosis Factor alpha (TNFa)

Size

£1,004.00

SKU:: T9160-36S
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Leu79-Leu234 from guinea pig TNF-a expressed in E. coli.
Clone:: 11D56
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Extra Details:: TNF-a, designated TNFSF1A, is a trimeric glycoprotein active in both membrane bound and secreted forms. TNF-a is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. TNF-a binds to TNF RI and TNF RII present on virtually all cell types where it triggers the activation of multiple signal transduction pathways and modulates the expression of a wide variety of genes. Applications: Suitable for use ELISA. Other applications not tested. Recommended Dilution: ELISA (Capture): 2-8ug/ml using T9160-36Q as the detection antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.