T9160-28B-ML650

Tumor Necrosis Factor alpha (TNFa) (MaxLight 650)

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£812.00

SKU:: T9160-28B-ML650
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Goat
Immunogen:: Highly purified (> 98%) murine TNFa.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Extra Details:: MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. Applications: Suitable for use in FLISA, Western Blot and Neutralization. Other applications have not been tested. Recommended Dilution: FLISA: 0.5ug/ml (100ul/well antibody solution). Detection of 0.2-0.4ng mTNFa/well. Western Blot: 0.10.2ug/ml. Detection limit is 1.5-3ng mTNFa/lane, under either reducing or non-reducing conditions. Neutralization: To yield one half maximal inhibition (ND50) of the biological activity of mTNF-alpha (0.25ng/ml), a concentration of 0.05-0.08ug/ml of this antibody is required. Optimal dilutions to be determined by the researcher. Reconstitution: Reconstitute in sterile water to a concentration of ≥ 0.2mg/ml. Storage and Stability: May be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA Note: Applications are based on unconjugated antibody.

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