T9160-13K

Tumor Necrosis Factor alpha (TNFa, TNF-alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, Cachectin, TNF, TNFA, TNFSF2) (Azide Free, Low Endotoxin)

Size

£748.00

SKU:: T9160-13K
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to mouse TNF alpha expressed in E. coli.
Clone:: 3H3094 (MP6-XT22)
Format:: Supplied as a liquid in PBS, pH 7.4. No preservatives added.
Extra Details:: TNF alpha is a 17kD cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26KD integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immuno-regulatory role. Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Functional Assays. Other applications not tested. Recommended Dilution: ELISA: ≤2ug/ml Immunohistochemistry (frozen): see references 13-19 Flow Cytometry: see references 6-12 Neutralization: see references 1 and 22-24 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.