T9160-13J

Tumor Necrosis Factor alpha (TNFa, TNF-alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, Cachectin, TNF, TNFA, TNFSF2)

Size

£650.00

SKU:: T9160-13J
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to mouse Tumor Necrosis Factor alpha (TNFa) expressed in E. coli.
Clone:: 3H3094 (MP6-XT22)
Format:: Supplied as a liquid in BBS, pH 8.2.
Extra Details:: Tumor Necrosis Factor alpha (TNFa) is a 17kD cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26kD integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immunoregulatory role. Applications: Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections ELISA: ≤ 2ug/ml Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul. Membrane permeabilization is required. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.