T9160-11H
Tumor Necrosis Factor alpha (TNFa)
Size
£716.00
- SKU:
- T9160-11H
- Purity:
- >90% (SDS-PAGE)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human TNFa.
- Clone:
- 6D815
- Format:
- Supplied as a liquid in PBS, pH 7.4.
- Extra Details:
- Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. Applications: Suitable for use in Flow Cytometry, ELISA and Neutralization. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ug/ml on PMBC. PMBC were incubated with PMA, ionomycin and with monensin to inhibit secretion. Incubation for 20 min with T9160-11H at 10ug/ml; stained with goat anti-mouse IgG-FITC secondary antibody for 15 min. ELISA: Use as a capture or detection antibody. Neutralization: Requires an incubation of antigen and antibody for 1 hour at 37°C or 16 hours at 4°C to completely neutralize TNFa cytolytic activity. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice