T9160-04A
Tumor Necrosis Factor alpha (TNFa)
Size
£812.00
- SKU:
- T9160-04A
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human TNF-a.
- Clone:
- CH8810
- Format:
- Supplied as a lyophilized powder in 0.01M PBS, pH 7.0. Reconstitute with 500ul sterile ddH2O.
- Extra Details:
- Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. Applications: Suitable for use in ELISA and Neutralization. Other applications not tested. Recommended Dilution: Neutralizing: Neutralizes TNF-alpha activity in vitro, 2-5ng of rTNF-alpha per ug of MAb. ELISA: Matched pair antibodies are available Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice