T9160-03N

Tumor Necrosis Factor alpha (TNF alpha, TNF-alpha, TNF a, TNFa, TNF-a, Cachectin, Differentiation Inducing Factor, DIF, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF Superfamily Member 2, TNFSF2)

Size

£876.00

SKU:: T9160-03N
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Clone:: T1
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Extra Details:: Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. Applications: Suitable for use in ELISA, Immunohistochemistry, Flow Cytometry, Western Blot, Immunoprecipitation and Neutralization. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Western Blot: 1:50 Immunohistochemistry: 1:50 Neutralization: For neutralization of biological acitivity in vitro, dilution must be determined according to the amount of TNF-alpha to be inactivated. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice