T9160-02E

Tumor Necrosis Factor alpha, Recombinant, Bovine (TNFa)

Size

£1,125.00

SKU:: T9160-02E
Molecular Weight:: 17.4
Purity:: ≥95% (SDS PAGE). Endotoxin: ≤0.06EU/ug.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in PBS. No preservative added. Reconstitute with 500ul sterile ddH2O.
Extra Details:: Recombinant protein corresponding to bovine Tumor Necrosis Factor alpha, expressed in Pichia pastoris Description: Bovine Tumor Necrosis Factor alpha (TNF alpha) is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes. TNF alpha is an important mediator of the bovine inflammatory response. Biological Activity: ED50 in the range of 10-25ng/ml, determined by measuring the cytolytic effect of recombinant bovine TNF alpha on murine WEHI-13VAR cells in the presence of Actinomycin D. Applications: Suitable for use in In ELISA and Functional assays. Other applications not tested. Storage and Stability: Lyophilized and reconstituted products may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.