TUC-4 Protein (TOAD, Turned On After Division, Ulip/CRMP-4, DRP-3, Dihydropyrimidinase-like 3) (Not for Export EU)

Size

£780.00

SKU:: T9157
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa499-511 of TUC-4a and aa612-624 of TUC-4b (YDGPVFDLTTTPK)
Format:: Supplied as a liquid in 0.09% sodium azide.
Extra Details:: TUC (TOAD [Turned On After Division]/ Ulip/CRMP) is a family of proteins that have emerged as strong candidates for a role in neuronal growth cone signaling. The TUC-4 family members reach their highest expression levels in early post-mitotic neurons during their peak periods of axonal growth. TUC-4 is not expressed in neural progenitors or adult neurons (with minor exception). Data suggests that TUC-4 plays a role in axonal outgrowth, pathfinding and possibly in the process that permits growing axons to choose proper routes and targets (4). The TUC-4 protein is expressed primarily within the cytoplasm of postmitotic neurons as they begin their migration out of the ventricular zone into the developing cortical plate (2). TUC-4 is expressed in neuronal cell bodies, neurites, and along the most distal regions of neuronal growth cones, including the lamellipodia and filopodia (3). Expression of TUC-4 is observed by day E12 in rats coincident with the expression of the early neuronal marker class III beta-tubulin (4). TUC-4 expression is rarely observed in neuronal cells expressing the mitotic marker PCNA (2). The expression of TUC-4 has been shown to be upregulated in response to NGF in rats (3) and in response to retinoic acid in a human neuroblastoma cell line (SMS-KCNR). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:2500-1:25,000 using akaline phosphatase Immunohistochemistry: 1:1000-1:5000 using PFA (DAB detection). Immunocytochemistry: 1:1000-5000 (DAB detection). Optimal dilutions to be determined by the researcher. Positive Control: Fetal brain/spinal cord tissue. Human neuroblastoma cell line SMS-KCNR or rat PC12 cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice