T9157-32
TBCA (Tubulin folding cofactor A)
Size
£484.00
- SKU:
- T9157-32
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa1-108 from human TBCA, expressed in E. coli.
- Clone:
- AT1A5
- Format:
- Supplied as a liquid In PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
- Extra Details:
- Tubulin folding cofactor A, also known as TBCA, is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin in a quasi-native confirmation. This protein is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunofluorescence (IC): 1;100 Flow Cytometry: 2-5ug/ml to label 1x10e6 cells Optimal dilutions to be determined by the researcher. Hybridoma: F0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice