T9156-65A

Tubulin Polymerization Promoting Protein (TPPP, TPPP1, TPPP/p25, p25alpha, p25, p24, Glycogen synthase kinase 3 (GSK3) inhibitor p24, Brain specific protein p25 alpha)

Size

£642.00

SKU:: T9156-65A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-DPSKDRAAKRLSLES of human TPPP.
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA (Peptide): 1:32000 Western Blot: 0.001-0.01ug/ml Immunohistochemistry (FFPE): 1-2ug/ml Human Cerebellum shows strong staining of axonal bundles through the granular layer. Requires HIER using sodium citrate buffer pH6.0 prior to staining. Immunohistochemistry (FFPE): 3.75ug/ml Human Cortex Requires HIER using sodium citrate buffer pH6.0 prior to staining. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after reciept. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.