T8669-70B
Trypsin-3 (PRSS3, Mesotrypsin)
Size
£761.00
- SKU:
- T8669-70B
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- NS0-derived recombinant protein corresponding to Val16-Ser247 from human Trypsin-3.
- Clone:
- 8B49
- Format:
- Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Trypsin-3, also known as mesotrypsin, is one of the three trypsins produced by the pancreas. It consists of a pro peptide (aa16-23) and a mature chain (aa24-247). Applications: Suitable for use in Neutralization and Western Blot. Other applications have not been tested. Recommended Dilutions: Neutralization: T8669-70B was preincubated with the enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50% of the proteolytic activity was inhibited by the antibody at approximately 0.15ug/ml(IC 50) under conditions in which the enzyme was present at 0.03ug/ml and the substrate concentration was 10uM. Considering the molecular masses of the enzyme (26kD) and the antibody (150kD), IC50 was achieved at approximately 1:1 molar ratio of the antibody to the enzyme. Optimal dilutions to be determined by the researcher. Endotoxin: < 0.1EU/ug (LAL) Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice