T8669-60B
Trypsin 1 (PRSS1)
Size
£812.00
- SKU:
- T8669-60B
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ala16-Ser247 from human Trypsin 1, expressed in NS0 cells.
- Clone:
- 8B48
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Trypsin 1, also known as cationic trypsinogen, contains a signal peptide (aa1-15), a pro region (aa16-23), and a mature chain (aa24-247). Trypsin 1 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. Its major physiologic function is to digest food and to activate other pro-enzymes by cleaving peptide bonds after arginine or lysine. Mutations in Trypsin 1 can cause hereditary pancreatitis. The amino acid sequence of human Trypsin 1 is 79% identical to that of mouse. Applications: Suitable for use in Western Blot, Immunoprecipitation and Direct ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1ug/ml. Recombinant Human Trypsin 1 under non-reducing conditions only Immunoprecipitation: 25ug/ml. Conditoined cell culture medium spiked with Recombinant Human Trypsin 1 Optimal dilutions to be determined by the researcher Hybridoma: NSO myeloma cells with spleen cells from BALB/c mice. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice