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T8669-05

Trypsin (Biotin)

Size

£889.00

SKU:
T8669-05
Purity:
Purified by delipidation, fractionation and ion exchange chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rabbit
Immunogen:
Trypsin from Bovine Pancreas.
Format:
Supplied as a liqud in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free). 0.01% sodium azide. Labeled with Biotin.
Extra Details:
Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. Trypsin is inhibited by organophosphorous compounds such as diisopropylfluorophosphate and porcine-derived inhibitors. Used in protein sequencing and tissue disassociation. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:5000-1:25,000 Western Blot: 1:500-1:3000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice