T8665-99X-ML550
TRP75, gp75 (Tyrosinase Related Protein 75) (BSA & Azide Free) (MaxLight 550)
Size
£931.00
- SKU:
- T8665-99X-ML550
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- SK-MEL-23 Melanoma cell line. MW of Antigen: 75kD. Cellular Localization: Cytoplasmic
- Clone:
- 3F388
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
- Extra Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. The tyrosinase family in vertebrates consists of three related melanogenic enzymes: tryrosinase, tyrosinase-related protein-1 (TRP-1) and tyrosinase-related protein-2 (TRP-2). These proteins control melanin production in pigment cells and play a crucial role in determining vertebrate coloration and are expressed by melanoma cells, normal melanocytes and nevi, but not carcinomas or sarcomas. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Positive Control: SK-MEL-23, A375 cells, Melanoma and Skin Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice