T8663

TrkA (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR)

Size

£1,010.00

SKU:: T8663
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Bacterially expressed purified protein corresponding to the entire extracellular domain of rat TrkA receptor.
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.09% sodium azide.
Extra Details:: TrkA has an apparent molecular weight 140kD and is a high affinity nerve growth factor (NGF). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-2.0ug/ml detected TrkA in RIPA lysates from human A431 cells, TrkA in mouse 3T3 and rat PC-12 cell lysates. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with T8663 (2ug/ml). Proteins were visualized using a IgG (HRP) Gt x Rb and a chemiluminescence detection system. Immunoprecipitation: 4ug immunoprecipitates TrkA from 500ug of PC-12 RIPA lysate. Immunohistochemistry (Paraffin): 1:200 detected TrkA in neurons of the hippocampal region and adrenal gland’s medulla. Requires HIER using sodium citrate buffer pH 6.0 prior to staining. Immunocytochemistry: 10ug/ml showed positive staining for TrkA in PC-12 cells fixed with 4% paraformaldehyde. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb Included Positive Antigen Control: A0001-50: Non-stimulated A431 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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