T8663-APC
TrkA (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR) (APC)
Size
£1,246.00
- SKU:
- T8663-APC
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Bacterially expressed purified protein corresponding to the entire extracellular domain of rat TrkA receptor.
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
- Extra Details:
- TrkA has an apparent molecular weight 140kD and is a high affinity nerve growth factor (NGF). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Paraffin): Requires HIER using sodium citrate buffer pH 6.0 prior to staining. Immunocytochemistry: Positive staining for TrkA in PC-12 cells fixed with 4% paraformaldehyde. Optimal dilutions to be determined by the researcher. Included Positive Antigen Control: A0001-50: Non-stimulated A431 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice