T8464-35D

TRIM (T Cell Receptor Interacting Molecule, T-cell Receptor-interacting Molecule, TCRIM, HSPC062, pp29/30, T Cell Receptor Associated Transmembrane Adaptor 1, T-cell Receptor-associated Transmembrane Adapter 1, TRAT1)

Size

£704.00

SKU:: T8464-35D
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa29-186 from human TRIM, intracellular domain.
Clone:: TRIM-04
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30kD protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear. Applications: Suitable for use in Western Blot, Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1-4ug/ml, intracellular staining Western Blot: Non-reducing conditions Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.