T8464-35B
TRIM (T Cell Receptor-Interacting Molecule)
Size
£825.00
- SKU:
- T8464-35B
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- E. coli-derived recombinant protein corresponding to Thr50-Asn186 from TRIM.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Human TRIM (T cell receptor–interacting molecule) (also trat1; T cell receptor-associated transmembrane adaptor 1, and pp29/30) is a 27-30kD, type III transmembrane protein, that is a member of the transmembrane adaptor protein (TRAP) family. It contains a short, 8 amino acid (aa) extracellular region, a 19 aa transmembrane region, and a 159 aa cytosolic tail. Its cytoplasmic tail contains several tyrosine motifs with the potential to bind to Src-homology 2 (SH2) domains of signaling proteins. TRIM is present in T cells and NK cells. Human TRIM shares 66% aa sequence identity with mouse TRIM. Applications: Suitable for use in Flow Cytometry, Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 25ug/ml use 10ul to label 10e6 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice