T8300-11A
Treponema pallidum (HRP) (Syphilis)
Size
£535.00
- SKU:
- T8300-11A
- Purity:
- Purified IgG fraction to a purity of > 95%.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Highly purified Treponema pallidum
- Format:
- Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.002% thimerosal. Labeled with horseradish peroxidase (HRP)
- Extra Details:
- Treponema pallidum is the causative agent of syphilis. It is a spirochete, a helical to sinusoidal bacterium with outer and cytoplasmic membranes, a thin peptidoglycan layer, and periplasmic flagella. Mechanisms of T. pallidum pathogenesis are poorly understood. No known virulence factors have been identified, and the outer membrane is mostly lipid with a paucity of proteins. Consequently, existing diagnostic tests for syphilus are suboptimal, and no vaccine against T. pallidum is available. TIGR sequenced the genome of T. pallidum subsp. pallidum (strain Nichols) by the whole genome random sequencing method. The T. pallidum genome is a circular chromosome of 1,138,006 base pairs containing a total of 1041 predicted open reading frames. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Titer: Indirect Immunofluorescence: >1:3,000 Non-reactive in RPR assay. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice