T8282-10B
TREM-2b (Trem2b, Triggering Receptor Expressed on Monocytes 2) (PE)
Size
£843.00
- SKU:
- T8282-10B
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- 4°C Do not freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- NS0-derived recombinant protein corresponding to mouse TREM-2b extracellular domain.
- Clone:
- 7H10
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- TREM-2 is an Ig superfamily cell surface receptor that activates a number of myeloid cell types. It is a member of a small gene family located on human chromosome 6p21 and mouse chromosome 17 in a region linked to the MHC. A single human TREM-2 gene has been described, however, two closely related orthologs were reported in mouse. The proteins differ by only three amino acids and were designated TREM-2a and TREM-2b. TREM-2 is type I transmembrane protein consisting of a single extracellular immunoglobulin (V-like) domain, a transmembrane domain with a positively charged lysine residue, and a short cytoplasmic tail. It associates with the signal adapter protein, DAP12, for signaling and function. DAP12 has a cytoplasmic ITAM that is phosphorylated upon ligand binding leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM-2 is expressed by immature monocyte-derived dendritic cells (DC), and expression is down-regulated upon activation of DC by microbial products and costimulatory signals. Ligation of TREM-2 on immature DC with anti-TREM-2 antibodies results in partial DC activation and the up-regulation of CCR7 and some co-stimulatory molecules. A role for TREM-2 in the functioning of osteoclasts and microglia is suggested by the discovery that homozygous loss-of-function mutations in either TREM-2 or DAP12 result in Nasu-Hakola disease characterized by a combination of presenile demetia and bone cysts. In vitro studies indicate that the differentiation of myeloid precursors into osteoclasts is dramatically impaired in TREM-2 deficient individuals. Applications: Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: Use 10ul to label 1x10e6 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice